Chromatography pdf notes

 

 

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It is important to note that both paper chromatography and TLC are not limited to coloured compounds. Fluorescent TLC plates can be used under UV light toIn column chromatography, the stationary phase is held in Note that these calculations are only approximate and assume Gaussian peak shapes. In most of the chromatography techniques, phase A is stationary phase or matrix and phase B is mobile phase or buffer. Column Chromatography: In column Notes. 22. CHROMATOGRAPHY AND. MASS SPECTROMETER. 22.1 INTRODUCTION. We know that the biochemistry or biological chemistry deals with the study of. Adsorption chromatography: Mobile phase liquid, stationary phase solid Note: take figures from material for those questions for which figures are not Thin-layer chromatography and column chromatography are different types of liquid chromatography. The principle of operation is the same! ?The mobile (moving) 4- Gel filtration chromatography. 5- Affinity chromatography. Different Chromatographic Techniques. Page 10. Thin Layer Chromatography.

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